Composite
Part:BBa_K2404014
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-10-16)
Luc+ gene under control of the LexA promoter
We generated a luciferase construct to test the efficacy of the tobacco leave expression system.
This construct contains the LexA promotor, the luciferase coding sequence and the Nos terminator.
This part is contained within the Phytobrick level 1 pGB-A1 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.
Sequence and Features
Sequence detail:
:
1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 15
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 15
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1171
Illegal BamHI site found at 1017 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 15
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 15
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |